New DNA methyltransferase M.AjnI from the bacterium Acinetobacter johnsonii R2 produces the 5′-m5CCWGG-3′ sequence

  • V. S. Dedkov
  • Published 2010 in Applied Biochemistry and Microbiology

Abstract

Optimum conditions for the activity of the new DNA methylase in cell lysate were determined. Methylation of DNAs of bacteriophages λ and T7 and plasmid pBR322 (dcm+) in the 5′-Cm5CWGG-3′ region blocked M.AjnI activity. The specificity of M.AjnI was determined using λ DNA methylated by this enzyme as well as computer modeling and data on the sensitivity of restriction endonucleases Mval, HinfI, and BstMAI to methylation.

DOI: 10.1134/S0003683810090061

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Cite this paper

@article{Dedkov2010NewDM, title={New DNA methyltransferase M.AjnI from the bacterium Acinetobacter johnsonii R2 produces the 5′-m5CCWGG-3′ sequence}, author={V. S. Dedkov}, journal={Applied Biochemistry and Microbiology}, year={2010}, volume={46}, pages={849-853} }