Tuberculosis (TB) remains a major global health problem, responsible for ill health among millions of people each year.One of the main problem of TB treatment is the resistance of Mycobacterium tuberculosis. Continuous efforts to develop new drugs to combat M. Tuberculosisare under way and bioactive compounds of natural origin, particularly from plants, are gaining significance. Bangle Rhizome (Zingiber cassumuna rRoxb.) has been used empirically as anti Mycobacterium tuberculosis drug. The aim of researchto determineanti M.tuberculosis Strain H37Rv and MDR activity of methanol extract fraction of bangle rhizome (Zingiber cassumunar roxb.). powdered Bangle Rhizome were macerated with hexane and methanol, respectively. Methanol extract then was fractionated by vacuum liquid chromatography. Anti Mycobacterium tuberculosis assay was done with Microscopic ObservationDrugSuspectibility (MODS) method and at final concentration at 100 ppm of fractions. Methanolextract of bangle rhizome resulting fivefactions (Fraction A,B,C,D and E). M. tuberculosis assay results show that the most active fraction is B. Chemical screening of fraction B using TLC visualization reagents indicated that fraction B contains flavonoids and terpenes. Keyword : Zingiber cassumunar Roxb., Mycobacterium tuberculosis, MODS.