New Colorimetric reaction for end-point, continuous-flow, and kinetic measurement of urea.

Abstract

A reaction of urea, o-phthalaldehyde and N-(1-naphthyl)ethylenediamine is described for measurement of urea by manual, continuous-flow, and kinetic methods. The continuous-flow system requires 25 mu-l of sample; 40 samples can be analyzed per hour. The kinetic method requires no enzymes, has no lag phase, and has good sensitivity. A major advantage of the reaction is that it occurs at a temperature of 37 degrees C or lower. The results obtained by all three methods agree well with those for a continuous-flow procedure in which diacetyl is a reagent.

6 Figures and Tables

0100200300'78'81'85'89'93'97'01'05'09'13'17
Citations per Year

635 Citations

Semantic Scholar estimates that this publication has 635 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Jung1975NewCR, title={New Colorimetric reaction for end-point, continuous-flow, and kinetic measurement of urea.}, author={Da Hee Jung and Harry Biggs and J Michael Erikson and P U Ledyard}, journal={Clinical chemistry}, year={1975}, volume={21 8}, pages={1136-40} }