Neutrophil chemotactic activity elaborated by human dental plaque

  title={Neutrophil chemotactic activity elaborated by human dental plaque},
  author={Jan Lindhe and Leif B Helld{\'e}n},
In the present investigation an in vivo method is described for studying the chemotactic activity elaborated by factors in human dental plaque. Plaque was sampled from the buccal and lingual surfaces of teeth from dental students, who had refrained from mechanical tooth cleansing during one 4 and one 8 day period. The plaque samples were suspended in 0.15 M NaCl, homogenized, and centrifuged at 12,100 × g for 30 minutes at 4°C. The plaque supernatant was then separated from the cellular pellet… CONTINUE READING