Neutralization of West Nile virus by cross-linking of its surface proteins with Fab fragments of the human monoclonal antibody CR4354.

@article{Kaufmann2010NeutralizationOW,
  title={Neutralization of West Nile virus by cross-linking of its surface proteins with Fab fragments of the human monoclonal antibody CR4354.},
  author={B{\"a}rbel Kaufmann and Matthew R Vogt and Jaap Goudsmit and Heather A. Holdaway and Anastasia A. Aksyuk and Paul R. Chipman and Richard J. Kuhn and Michael S Diamond and Michael G. Rossmann},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2010},
  volume={107 44},
  pages={18950-5}
}
Many flaviviruses are significant human pathogens, with the humoral immune response playing an essential role in restricting infection and disease. CR4354, a human monoclonal antibody isolated from a patient, neutralizes West Nile virus (WNV) infection at a postattachment stage in the viral life-cycle. Here, we determined the structure of WNV complexed with Fab fragments of CR4354 using cryoelectron microscopy. The outer glycoprotein shell of a mature WNV particle is formed by 30 rafts of three… CONTINUE READING