Neutral red uptake assay for the estimation of cell viability/cytotoxicity

@article{Repetto2008NeutralRU,
  title={Neutral red uptake assay for the estimation of cell viability/cytotoxicity},
  author={Guillermo Repetto and Ana del Peso and Jorge L. Zurita},
  journal={Nature Protocols},
  year={2008},
  volume={3},
  pages={1125-1131}
}
The neutral red uptake assay provides a quantitative estimation of the number of viable cells in a culture. [] Key Method Cells are seeded in 96-well tissue culture plates and are treated for the appropriate period. The plates are then incubated for 2 h with a medium containing neutral red. The cells are subsequently washed, the dye is extracted in each well and the absorbance is read using a spectrophotometer. The procedure is cheaper and more sensitive than other cytotoxicity tests (tetrazolium salts…

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