Neutral red uptake assay for the estimation of cell viability/cytotoxicity

  title={Neutral red uptake assay for the estimation of cell viability/cytotoxicity},
  author={Guillermo Repetto and Ana del Peso and Jorge L. Zurita},
  journal={Nature Protocols},
The neutral red uptake assay provides a quantitative estimation of the number of viable cells in a culture. [] Key Method Cells are seeded in 96-well tissue culture plates and are treated for the appropriate period. The plates are then incubated for 2 h with a medium containing neutral red. The cells are subsequently washed, the dye is extracted in each well and the absorbance is read using a spectrophotometer. The procedure is cheaper and more sensitive than other cytotoxicity tests (tetrazolium salts…

Assaying Cellular Viability Using the Neutral Red Uptake Assay.

The present protocol describes the neutral red uptake assay using the human hepatoma cell line HepG2, which is often employed as an alternative in vitro model for human hepatocytes, and the cytotoxicity of acetaminophen and acetyl salicylic acid is assessed.

Chapter 1 In Vitro Cytotoxicity and Cell Viability Assays : Principles , Advantages , and Disadvantages

In this chapter, information is given about in vitro cytotoxicity and viability assays, these assays will be classified and their advantages and disadvantages will be emphasized.

In Vitro Cytotoxicity and Cell Viability Assays: Principles, Advantages, and Disadvantages

In this chapter, information is given about in vitro cytotoxicity and viability assays, these assays will be classified and their advantages and disadvantages will be emphasized.

A simplified colorimetric method for rapid detection of cell viability and toxicity in adherent cell culture systems.

The proposed JG-B cell viability assay does not require a solubilization/extraction step, and hence follows a much simpler and time-efficient protocol suitable for high-throughput analysis of cell viability in anchorage-dependent cell culture models.

The neutral red assay can be used to evaluate cell viability during autophagy or in an acidic microenvironment in vitro

The modified neutral red assay detected cell viability accurately over a range of pHe as demonstrated by its correlation with induction of apoptosis and autophagy, and is effective for evaluating the effect of chemotherapeutic agents on cell viability under acidic or hypoxic conditions.

Development of a quantitative cytotoxicity assay using mouse lymphoma TK cells.

  • Xuemei LiuFan Zhang R. Rose
  • Biology, Medicine
    Toxicology in vitro : an international journal published in association with BIBRA
  • 2017

Reduction of MTT to Purple Formazan by Vitamin E Isomers in the Absence of Cells.

It is demonstrated that the vitamin E isomers α-β-γ-δ-tocotrienols and α-tocopherol were able to reduce MTT into a formazan product, despite the absence of living cells, suggesting that the MTT assay is not suitable for studying the proliferative effects of vitaminE isomers on cell growth.

Application of Neutral Red Uptake Assay Using EPC Cells as an Alternative to the Fish Acute Toxicity Test for Pesticide

The results suggested that the potential of EPC cell viability assay as an alternative to the fish acute toxicity test due to their good correlation and NRU assay is expected to serve as a useful tool for predicting acute fish lethality for pesticides if further studies with a large set of pesticides are conducted.

Validation of eGFP fluorescence intensity for testing in vitro cytotoxicity according to ISO 10993-5.

Measurement of the eGFP signal of transfected NIH-3T3 fibroblasts with the results of the MTT test is validated to provide a test procedure that is very close to the ISO 10993-5 but has the advantage of not relying on the addition of dye.



Applications of the Neutral Red Cytotoxicity Assay to In Vitro Toxicology

The neutral red in vitro cytotoxicity assay was developed and has been used to determine the relative acute cytotoxicities of a broad spectrum of chemical test agents, to establish structure-toxicity relationships for series of related chemicals, to study metabolism-mediated cytot toxicity and to evaluate interactions between combinations of test agents.

Comparisons of two in vitro cytotoxicity assays-The neutral red (NR) and tetrazolium MTT tests.

A simple quantitative procedure using monolayer cultures for cytotoxicity assays (HTD/NR-90)

A sensitive quantitative procedure for assaying viable cells in monolayer cultures is described, conveniently carried out within the same culture, and standardized for use with 96-well microtiter plates and automatic reading with a Dynatech spectrophotometric microplate reader.

Sulforhodamine B colorimetric assay for cytotoxicity screening

The sulforhodamine B (SRB) assay is used for cell density determination, based on the measurement of cellular protein content, which is an efficient and highly cost-effective method for screening.

Automated colorimetric assay for T cell cytotoxicity.

A colorimetric method has been developed for detecting the lysis of target cells by cytotoxic T lymphocytes (Tc), which is substantially more sensitive than the 51Cr release assay and can be used to detect alloreactive Tc cells and H-2-restricted T c cells against viruses, haptens and minor-H antigens.

Neutral Red Uptake, Cellular Growth and Lysosomal Function: In Vitro Effects of 24 Metals

In this adaptation of the standard neutral red cytotoxicity assay, the results are expressed relative to cell culture protein content to avoid misinterpretation due to the influence on cell proliferation of the chemicals tested.

A semi-automated neutral red based chemosensitivity assay for drug screening

The assay technique was determined to be capable of detecting antineoplastic compounds operating by a wide variety of mechanisms, and in good agreement with results from clonogenic assays using similar drug treatment conditions.

Quantification of adherent and nonadherent cells cultured in 96-well plates using the supravital stain neutral red.

This assay offers a reliable and flexible tool to determine both stimulatory and inhibitory effects of hormones, cytokines, and drugs on cell growth or to study the effects on cell viability, making it ideal for screening of large numbers of samples.

Automated fluorometric assay for T cell cytotoxicity.

Comparative Cytotoxicity of Alachlor on RTG-2 Trout and SH-SY5Y Human Cells

The proposed battery approach is an effective screening tool for the safety assessment of environmental contaminants as a complement to fish and animal toxicity procedures.