Neutral endopeptidase from nuchal ligament of fetal calves.

  title={Neutral endopeptidase from nuchal ligament of fetal calves.},
  author={A. R. Johnson and Larry D. Gray and Erik B. Youngblood and Janet R. Sullivan},
  journal={Journal of cellular biochemistry},
  volume={43 3},
The nuchal ligament of unborn calves contains a neutral endopeptidase that is biochemically and immunologically similar to the neutral endopeptidase (NEP), or enkephalinase, from human kidney. Enzymatic activity was inhibited more than 90% by phosphoramidon (1 microM). The specific activity in membrane fractions, as determined by hydrolysis of the dansylated substrate, DAPGN, was similar in tissue from fetuses of gestational ages ranging from 100 to 280 days. NEP activity in adult ligament… CONTINUE READING