Neurosteroids act on recombinant human GABAA receptors

  title={Neurosteroids act on recombinant human GABAA receptors},
  author={G. Puia and M. Santi and S. Vicini and D. Pritchett and E. Costa},
  • G. Puia, M. Santi, +2 authors E. Costa
  • Published 1990
  • Chemistry, Medicine
  • Neuron
  • The endogenous steroid metabolites 3 alpha,21dihydroxy-5 alpha-pregnan-20-one and 3 alpha-hydroxy-5 alpha-pregnan-20-one potentiate GABA-activated Cl- currents recorded from a human cell line transfected with the beta 1, alpha 1 beta 1, and alpha 1 beta 1 gamma 2 combinations of human GABAA receptor subunits. These steroids are active at nanomolar concentrations in potentiating GABA-activated Cl- currents and directly elicit bicuculline-sensitive Cl- currents when applied at micromolar… CONTINUE READING
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