Neurons cultured from developing rat brain attach and spread preferentially to laminin.

@article{Liesi1984NeuronsCF,
  title={Neurons cultured from developing rat brain attach and spread preferentially to laminin.},
  author={P{\"a}ivi Liesi and Doris Dahl and Antti Vaheri},
  journal={Journal of neuroscience research},
  year={1984},
  volume={11 3},
  pages={241-51}
}
Neurons, mechanically dissociated from newborn rat brain and identified by immunostaining for neurofilaments, attached preferentially to laminin-coated coverslips without need of an underlying glial monolayer. The most extensive neurite outgrowth was seen when 20-30 micrograms/ml of laminin was used to coat the coverslips. Higher concentrations of laminin (greater than 30 micrograms/ml) supported single neurons to spread on the coverslips. Fibronectin coating of the coverslips allowed glial… CONTINUE READING

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