Neurons and glia arise from a common progenitor in chicken optic tectum: demonstration with two retroviruses and cell type-specific antibodies.

@article{Galileo1990NeuronsAG,
  title={Neurons and glia arise from a common progenitor in chicken optic tectum: demonstration with two retroviruses and cell type-specific antibodies.},
  author={Deni S Galileo and Grace E. Gray and Geoffrey C. Owens and John Majors and Joshua R Sanes},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1990},
  volume={87 1},
  pages={458-62}
}
We used a recombinant retrovirus to study cell lineage in the chicken optic tectum. The virus inserts the Escherichia coli lacZ (beta-galactosidase) gene into the genome of an infected cell; a histochemical stain marks the progeny of infected cells with a blue precipitate. We had previously shown that individual clones frequently contain diverse neuronal types. Now we asked whether individual clones contain glia as well as neurons. To this end, we constructed a virus in which lacZ is fused to a… CONTINUE READING