Neuronal nitric oxide synthase localizes through multiple structural motifs to the sarcolemma in mouse myotubes.

@article{Abdelmoity2000NeuronalNO,
  title={Neuronal nitric oxide synthase localizes through multiple structural motifs to the sarcolemma in mouse myotubes.},
  author={A Abdelmoity and Roanna C Padre and K E Burzynski and James T Stull and Kim S. Lau},
  journal={FEBS letters},
  year={2000},
  volume={482 1-2},
  pages={65-70}
}
In skeletal muscle, neuronal nitric oxide synthase is localized at the sarcolemma in association with the dystrophin glycoprotein complex (DGC). The nNOS N-terminal 231 amino acids comprise a PDZ domain (residues 1-100) and a beta-hairpin finger loop (residues 101-130) which binds alpha-syntrophin located in the DGC. Endogenous nNOS and GFP-tagged nNOS localize to the sarcolemma in mouse C2C12 myotubes. Expression of GFP-tagged nNOS domains in C2C12 myotubes reveals that the PDZ domain and the… CONTINUE READING