Neuron-specific human glutamate transporter: molecular cloning, characterization and expression in human brain

@article{Shashidharan1994NeuronspecificHG,
  title={Neuron-specific human glutamate transporter: molecular cloning, characterization and expression in human brain},
  author={Pullanipally Shashidharan and George W. Huntley and Thomas D. Meyer and John H. Morrison and Andreas Plaitakis},
  journal={Brain Research},
  year={1994},
  volume={662},
  pages={245-250}
}
Cloning and expression of a neuronal rat brain glutamate transporter.
Possible expression of functional glutamate transporters in the rat testis.
TLDR
Results suggested that particular subtypes of central Glu transporters for the regulation of extracellular Glu concentrations in the rat testis could be constitutively and functionally expressed.
EAAC 1 , a High-affinity Glutamate Transporter , is Localized to Astrocytes and Gabaergic Neurons besides Pyramidal Cells in the Rat Cerebral Cortex
TLDR
The localization of EAAC1 may explain the pathological symptoms that follow EAAC knockout (seizures and mild toxicity), as seizures could be due to the loss ofEAAC1-mediated fine regulation of neuronal excitability at axodendritic and axospinous synapses, whereas the mild toxicity may be related to the functional inactivation of astrocytic EAAC 1.
High affinity glutamate transporters: regulation of expression and activity.
TLDR
Four homologous types of Na/K-dependent high affinity glutamate transporters, glutamate/aspartate transporter, glutamate transporter 1, excitatory amino acid carrier 1, and excitatories amino acid transporter 4, have recently been cloned and were assigned to a separate gene family, together with two neutral amino acid carriers.
Molecular biology of mammalian plasma membrane amino acid transporters.
TLDR
The tissue expression, transport characteristics, structure-function relationship, and the putative physiological roles of four amino acid transporters belonging to four protein families are described.
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References

SHOWING 1-10 OF 13 REFERENCES
Cloning and expression of a rat brain L-glutamate transporter
TLDR
An antibody against a glial L-glutamate transporter from rat brain is used to isolate a complemen-tary DNA clone encoding this transporter, which predicts a protein of 573 amino acids with 8–9 putative transmembrane α-helices that seems to be a member of a new family of transport molecules.
Structure, expression, and functional analysis of a Na(+)-dependent glutamate/aspartate transporter from rat brain.
TLDR
The structure and function of a 66-kDa glycoprotein that was purified from rat brain and identified as an L-glutamate/L-aspartate transporter (GLAST) are described and data suggest that GLAST may be involved in the regulation of neurotransmitter concentration in central nervous system.
Primary structure and functional characterization of a high-affinity glutamate transporter
TLDR
A complementary DNA encoding an electrogenic Na+ but not Cl−-dependent high-affinity glutamate transporter (named EAAC1) is isolated from rabbit small intestine by expression in Xenopus oocytes and transcripts are found in specific neuronal structures in the central nervous system as well as in the small intestine, kidney, liver and heart.
Decreased glutamate transport by the brain and spinal cord in amyotrophic lateral sclerosis.
TLDR
ALS is associated with a defect in high-affinity glutamate transport that has disease, region, and chemical specificity and could lead to neurotoxic levels of extracellular glutamate and thus be pathogenic in ALS.
The glutamatergic nerve terminal.
TLDR
As well as playing the dominant role in fast information transfer, glutamate is of particular interest in view of its involvement in current models of memory and learning and because a pathological release of glutamate in brain ischaemia is neurotoxic and a major contributor to the damage caused under these conditions.
Differential effects of monocular deprivation on glutamic acid decarboxylase and type II calcium-calmodulin-dependent protein kinase gene expression in the adult monkey visual cortex [published erratum appears in J Neurosci 1991 May;11(5):following Table of Contents]
TLDR
In situ hybridization in the cortex from animals that had been monocularly deprived revealed enhanced CaM II kinase mRNA levels in deprived-eye columns of layer IVC and, associated with the deprived eye, cytochrome oxidase-stained periodicities in other layers.
Limbic seizures increase neuronal production of messenger RNA for nerve growth factor.
TLDR
In situ hybridization and nuclease protection analyses demonstrate that limbic seizures dramatically increase the amount of messenger RNA for NGF in the neurons of the hippocampal dentate gyrus within 1 hour of seizure onset and in broadly distributed neocortical and olfactory forebrain neurons some hours later.
Abnormal glutamate metabolism in amyotrophic lateral sclerosis
TLDR
Oral glutamate loading resulted in significantly greater elevations in the plasma glutamate and aspartate levels in the ALS patients than in the controls, suggesting a systemic defect in the metabolism of this amino acid may underlie primary ALS.
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