Neuron Neurotechnique Supraresolution Imaging in Brain Slices using Stimulated-Emission Depletion Two-Photon Laser Scanning Microscopy

@inproceedings{Ding2009NeuronNS,
  title={Neuron Neurotechnique Supraresolution Imaging in Brain Slices using Stimulated-Emission Depletion Two-Photon Laser Scanning Microscopy},
  author={Jun B Ding and Kevin T. Takasaki and Bernardo L. Sabatini},
  year={2009}
}
Two-photon laser scanning microscopy (2PLSM) has allowed unprecedented fluorescence imaging of neuronal structure and function within neural tissue. However, the resolution of this approach is poor compared to that of conventional confocal microscopy. Here, we demonstrate supraresolution 2PLSM within brain slices. Imaging beyond the diffraction limit is accomplished by using nearinfrared (NIR) lasers for both pulsed two-photon excitation and continuous wave stimulated emission depletion (STED… CONTINUE READING
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