NeuroD2 regulates the development of hippocampal mossy fiber synapses

Abstract

The assembly of neural circuits requires the concerted action of both genetically determined and activity-dependent mechanisms. Calcium-regulated transcription may link these processes, but the influence of specific transcription factors on the differentiation of synapse-specific properties is poorly understood. Here we characterize the influence of NeuroD2, a calcium-dependent transcription factor, in regulating the structural and functional maturation of the hippocampal mossy fiber (MF) synapse. Using NeuroD2 null mice and in vivo lentivirus-mediated gene knockdown, we demonstrate a critical role for NeuroD2 in the formation of CA3 dendritic spines receiving MF inputs. We also use electrophysiological recordings from CA3 neurons while stimulating MF axons to show that NeuroD2 regulates the differentiation of functional properties at the MF synapse. Finally, we find that NeuroD2 regulates PSD95 expression in hippocampal neurons and that PSD95 loss of function in vivo reproduces CA3 neuron spine defects observed in NeuroD2 null mice. These experiments identify NeuroD2 as a key transcription factor that regulates the structural and functional differentiation of MF synapses in vivo.

DOI: 10.1186/1749-8104-7-9

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@inproceedings{Wilke2011NeuroD2RT, title={NeuroD2 regulates the development of hippocampal mossy fiber synapses}, author={Scott A. Wilke and Benjamin J Hall and Joseph K. Antonios and Laura A DeNardo and Stefanie Otto and Bo Yuan and Fading Chen and Elissa M. Robbins and Katie Tiglio and Megan E Williams and Zilong Qiu and Thomas Biederer and Anirvan Ghosh}, booktitle={Neural Development}, year={2011} }