Neuraminidase activity assays for monitoring MDCK cell culture derived influenza virus.

@article{Nayak2004NeuraminidaseAA,
  title={Neuraminidase activity assays for monitoring MDCK cell culture derived influenza virus.},
  author={Deba Prasad Nayak and Udo Reichl},
  journal={Journal of virological methods},
  year={2004},
  volume={122 1},
  pages={9-15}
}
Three assay methods were investigated for monitoring the time-course of neuraminidase (NA) activity of tissue culture derived equine influenza A virus from large-scale microcarrier cultivation and several steps of downstream processing required for the production of inactivated vaccines. Measurements of neuraminidase activity by a thiobarbituric acid (TBA) and a fluorometric method using Amplex Red as a fluorogen (FL-AR) did not correlate with the increase of hemagglutinin (HA) during virus… CONTINUE READING

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