Neural tube and notochord promote in vitro myogenesis in single somite explants.

@article{Stern1995NeuralTA,
  title={Neural tube and notochord promote in vitro myogenesis in single somite explants.},
  author={Henrietta Stern and Stephen D. Hauschka},
  journal={Developmental biology},
  year={1995},
  volume={167 1},
  pages={87-103}
}
We have developed an in vitro assay for examining the neural tube/notochord influence on somite myogenesis. No cells committed to myogenesis (operationally defined as immunopositive staining for myosin heavy chain after a 48-hr culture period) are present in the most caudal somites (I-V) of stage 8-11 chicken embryos, whereas by stage 14-15, the most caudal somites occasionally produce differentiated muscle cells when cultured alone. To test for responsiveness to neural tube, one somite of each… CONTINUE READING

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