Neural cell adhesion molecule in normal, denervated, and myopathic human muscle

  title={Neural cell adhesion molecule in normal, denervated, and myopathic human muscle},
  author={Neil R. Cashman and Jonathan Covault and Robert L. Wollman and Joshua R. Sanes},
  journal={Annals of Neurology},
The neural cell adhesion molecule (N‐CAM) is a cell‐surface glycoprotein that may mediate some intercellular adhesive interactions in the nervous system. In adult rat muscle, N‐CAM is concentrated near neuromuscular junctions and on satellite cells, but is nearly undetectable in nonsynaptic portions of myofibers. However, N‐CAM is abundant throughout myofibers in denervated and regenerating muscles. Using affinity‐purified antibodies to N‐CAM, we were able to demonstrate a similar distribution… 

Regenerating and denervated human muscle fibers and satellite cells express neural cell adhesion molecule recognized by monoclonal antibodies to natural killer cells

The study concludes that the commercially available monoclonal antibodies to NK cells, Leu‐19 and NKH‐1, are immunocytochemical markers for the satellite cells and the regenerating or newly denervated muscle fibers complementing conventional techniques in the diagnosis of patients with neuromuscular disorders.

Expression pattern of M‐cadherin in normal, denervated, and regenerating mouse muscles

It is demonstrated that Mcad is present in satellite cells in normal muscle, and enhanced staining at sites of contact between satellite cells and the parent muscle fiber suggests an additional, spatially restricted expression of Mcad in muscle fibers.

CD24, a signal-transducing molecule expressed on human B lymphocytes, is a marker for human regenerating muscle

Investigation of the expression of the CD24 molecule in developing nerve and muscle, normal adult and various diseased human muscles, strongly suggests that regenerative and fetal myotubes are different with respect to theCD24 molecule expression.

N-CAM is expressed in mature extraocular muscles in a pattern conserved among three species.

It is postulate that N-CAM expression may at least be a marker for susceptibility to, if not play a clinically significant role in, the etiology and pathobiology of this disease.

Fibre type specific expression of Leu19-antigen and N-CAM in skeletal muscle in various stages after experimental denervation

Leu-19 antigen, which seems to be identical with neural cell adhesion molecule (N-CAM), plays a major role in the innervation of muscle cells, and in adult muscle appears after denervation and during

Expression of Leu‐19 (CD56, N‐CAM) and nitric oxide synthase (NOS) I in denervated and reinnervated human skeletal muscle

It is suggested that innervation is an important factor for selective gene expression and positioning of NOS I and N‐CAM in skeletal muscle and gives practical information for the assessment of the phase and developmental stage of the denervation and reinnervation process.

Anti‐NKH‐1 antibody specifically stains unmyelinated fibres and non‐myelinating Schwann cell columns in humans

Investigation of nerve biopsy samples from patients with normal nerve biopsies and a variety of peripheral neuropathies showed that the anti‐NKH‐1 antibody recognizes an isoform of the neural cell adhesion molecule (N‐CAM) that is involved in cell‐cell interactions during embryonic stages and has been detected in skin autonomic nerves.



Distribution of N-CAM in synaptic and extrasynaptic portions of developing and adult skeletal muscle

The times and places at which N-CAM appears are consistent with its playing several distinct roles in myogenesis, synaptogenesis, and synaptic maintenance, including alignment of secondary along primary myot tubes, early interactions of axons with myotubes, and adhesion of Schwann cells to nerve terminals.

Neural cell adhesion molecule is on embryonic muscle cells and mediates adhesion to nerve cells in vitro

It is reported here that N-CAM antigenic determinants are also present on chick embryo skeletal muscle cells, suggesting that the N- CAM adhesion system can facilitate nerve-muscle as well as nerve-nerve interactions in vitro.

N-CAM at the vertebrate neuromuscular junction

The findings indicate that, in muscle, in addition to chemical modulation, cell-surface modulation of N-CAM occurs both in amount and distribution during embryogenesis and in response to denervation.

Specific regulation of N‐CAM/D2‐CAM cell adhesion molecule during skeletal muscle development.

N‐CAM/D2-CAM expression is temporally regulated and is expressed only at times of synapse formation consistent with the idea that it may be involved in early nerve‐muscle interactions.

Individual neural cell types express immunologically distinct N-CAM forms

A unique form of rat N-CAM recognized by Mab 3G6.41 is found on some but not all neuronal, glial, and muscle cells.

Antibodies that bind specifically to synaptic sites on muscle fiber basal lamina

Details of staining patterns, differential susceptibility of antigens to proteolysis, and adsorption experiments showed that the antibodies define at least three different determinants that are present in synaptic but not extrasynaptic BL.

Developmental biology of a neural cell adhesion molecule

Experiments in vivo indicate that N-CAM-mediated adhesion is essential to the orderly assembly of the visual system.

Topographic mapping of motor pools onto skeletal muscles

  • M. B. LaskowskiJ. Sanes
  • Biology
    The Journal of neuroscience : the official journal of the Society for Neuroscience
  • 1987
It is suggested that segmental ordering of axons in the nerve, axonal guidance at branch points in the nerves, and positional labels within the muscle may all contribute to the rostrocaudal mapping of motor pools onto muscle.