Neural and non-neural activation of electrogenic secretion by 5-hydroxytryptamine in the rat ileum in vitro.

@article{Rolfe1998NeuralAN,
  title={Neural and non-neural activation of electrogenic secretion by 5-hydroxytryptamine in the rat ileum in vitro.},
  author={Vivien E. Rolfe and R. J. Levin},
  journal={Acta physiologica Scandinavica},
  year={1998},
  volume={162 4},
  pages={
          469-74
        }
}
5-hydroxytryptamine (5-HT) stimulates electrogenic Cl- secretion in rat ileum stripped of its outer smooth musculature and myenteric plexus. The myenteric plexus, however, is a site of 5-HT synthesis in the gut, and the plexus mediates electrogenic ion secretion activated by luminal enterotoxin STa and taurocholate. Thus, we investigated the role of the myenteric plexus in 5-HT-induced electrogenic secretion in vitro by measuring short-circuit current (Isc, microamps) with voltage-clamp… 

Nitric oxide is a neurotransmitter in the chloride secretory response to serotonin in rat colon.

The ability of exogenous NO to induce a change in circuit current in the presence of tetrodotoxin suggests that NO is a final neurotransmitter in this neural-mucosal reflex and therefore acts directly on the enterocyte to induce secretion.

Vasoactive intestinal peptide is a neuropeptide mediator of the secretory response to serotonin in rat.

Activation of the neural 5-HT(3) receptor by 2-Me-5-HT induces a secretory response in rat colon that is inhibited by a VIP receptor antagonist, and VIP is a likely nonadrenergic, noncholinergic neurotransmitter in this pathway.

Inhibition of neural nitric oxide synthase attenuates the chloride secretory response to stroking in human jejunum.

The significantly reduced DeltaI(sc) in the group pretreated with the neural nitric oxide synthase inhibitor suggests that nitricoxide liberated from enteric neurons participates in the chloride secretory response to stroking in human jejunum in vitro.

Effects of 5-hydroxytryptamine on the short-circuit current across the small intestine of the gerbil (Gerbillus cheesmani) in different dietary states.

The results indicated that both starvation and undernourishment increase that response to 5-HT and such increases were TTX-sensitive and both chloride- and bicarbonate-dependent.

Functional characterization of serotonin receptor subtypes in human duodenal secretion.

5-HT induced a dose-dependent and bumetanide-sensitive short-circuit current, which was independent of the presence of Helicobacter pylori infection, which indicates that in human duodenum 5-HT is a potent stimulator of bumetanical-sensitive secretion.

5-HT induces duodenal mucosal bicarbonate secretion via cAMP- and Ca2+-dependent signaling pathways and 5-HT4 receptors in mice.

It is demonstrated that 5-HT regulates DMBS via both cAMP- and Ca(2+)-dependent signaling pathways and 5- HT(4) receptors located in the duodenal mucosa and/or epithelial cells.

Changes in 5-HT-mediated pathways in radiation-induced attenuation and recovery of ion transport in rat colon.

Investigation of the role of both neural and nonneural 5-hydroxytryptamine (5-HT)-mediated pathways in radiation-induced attenuation and recovery of colonic secretory function concluded that the recovery of Colonic transport is associated with additional 5-HT(3)- mediated pathways, probably in combination with 5- HT(4) receptors.

Electrogenic Ion Transport in Mammalian Colon Involves an Ammonia-Sensitive Apical Membrane K+ Conductance

Ammonia may be an endogenous regulator of colonic water and salt secretion and Apical K+ channels may be involved in the regulation of cAMP-stimulated Cl− secretion in mammalian colon.

Inhibiting Inducible Nitric Oxide Synthase in Enteric Glia Restores Electrogenic Ion Transport in Mice With Colitis.

Inhibition of enteric glial function in vivo reduced the severity of trinitrobenzene sulfonic acid- or dextran sodium sulfate-induced colitis and associated bacterial translocation and contributed to the dysregulation of intestinal ion transport in mice with colitis.

References

SHOWING 1-10 OF 17 REFERENCES

5-hydroxytryptamine and cholera secretion. Physiological and pharmacological studies in cats and rats.

The intestinal secretion evoked by close intra-arterial infusion of 5-hydroxytryptamine (5-HT) in cats was inhibited by tetrodotoxin, a drug abolishing action potentials, which strongly indicate that 5-HT-induced secretion is, at least in part, neurally mediated.

Action of 5‐hydroxytryptamine on intestinal ion transport in the rat.

Experiments in which the intestinal villi or crypts were subjected to preferential damage suggested that 5‐HT primarily produced its response at the crypt cell level, and the 5‐ HT response in vivo was unaffected by atropine, cyproheptadine, propranolol and hexamethonium.

Enterotoxin Escherichia coli STa activates a nitric oxide‐dependent myenteric plexus secretory reflex in the rat ileum.

Mucosally added enterotoxin Escherichia coli STa increased electrogenic Cl‐ secretion across intact rat ileum in vitro by activating a capsaicin‐sensitive, nitric oxide‐dependent myenteric plexus‐mediated secretory reflex, and suppression by L‐NAME of STa induced ileal fluid secretion in vivo probably involves the inhibition of this reflex.

5-Hydroxytryptamine and cholera secretion: a histochemical and physiological study in cats.

A hypothesis is presented for explaining the possible role of the enterochromaffin cells in the pathophysiology of cholera secretion, where a diminished 5-HT content was associated with a decreased rate of fluid absorption or an increased rate of secretion.

Characterization and localization of a peripheral neural 5- hydroxytryptamine receptor subtype (5-HT1P) with a selective agonist, 3H-5-hydroxyindalpine

5-OHIP is an agonist at peripheral neural 5-HT1P receptors and can be used to label these receptors selectively outside the brain, and is suggested that 5- HT1P receptor may be found on subtypes of primary afferent nerve fibers.

Identification and stimulation by serotonin of intrinsic sensory neurons of the submucosal plexus of the guinea pig gut: activity- induced expression of Fos immunoreactivity

The data suggest that intrinsic primary afferent neurons are located in the submucosal plexus, project to the myenteric plexi, and are activated by 5-HT acting on the 5- HT1P receptor subtype, which is mediated by the enteric nervous system.

Enteric receptors for 5-hydroxytryptamine