NeuBtracker—imaging neurobehavioral dynamics in freely behaving fish

Abstract

A long-standing objective in neuroscience has been to image distributed neuronal activity in freely behaving animals. Here we introduce NeuBtracker, a tracking microscope for simultaneous imaging of neuronal activity and behavior of freely swimming fluorescent reporter fish. We showcase the value of NeuBtracker for screening neurostimulants with respect to their combined neuronal and behavioral effects and for determining spontaneous and stimulus-induced spatiotemporal patterns of neuronal activation during naturalistic behavior.

DOI: 10.1038/nmeth.4459

Cite this paper

@article{Symvoulidis2017NeuBtrackerimagingND, title={NeuBtracker—imaging neurobehavioral dynamics in freely behaving fish}, author={Panagiotis Symvoulidis and Antonella Lauri and Anca Stefanoiu and Michele Cappetta and Steffen Schneider and Hongbo Jia and Anja Stelzl and Maximilian Koch and C. Cruz Perez and Ahne Myklatun and Sabine Renninger and Andriy Chmyrov and Tobias Lasser and Wolfgang Wurst and Vasilis Ntziachristos and Gil G. Westmeyer}, journal={Nature Methods}, year={2017}, volume={14}, pages={1079-1082} }