Network rigidity and metabolic engineering in metabolite overproduction

@article{Stephanopoulos1991NetworkRA,
  title={Network rigidity and metabolic engineering in metabolite overproduction},
  author={Gregory Stephanopoulos and JJ Vallino},
  journal={Science},
  year={1991},
  volume={252},
  pages={1675 - 1681}
}
In order to enhance the yield and productivity of metabolite production, researchers have focused almost exclusively on enzyme amplification or other modifications of the product pathway. However, overproduction of many metabolites requires significant redirection of flux distributions in the primary metabolism, which may not readily occur following product deregulation because metabolic pathways have evolved to exhibit control architectures that resist flux alterations at branch points. This… 
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