Nested polymerase chain reaction for detection of Legionella pneumophila in water.

@article{Cataln1994NestedPC,
  title={Nested polymerase chain reaction for detection of Legionella pneumophila in water.},
  author={Vicente Catal{\'a}n and Carmen Junceda Moreno and M A Das{\'i} and Cristi{\'a}n Mu{\~n}oz and David Apraiz},
  journal={Research in microbiology},
  year={1994},
  volume={145 8},
  pages={603-10}
}
A highly sensitive nested polymerase chain reaction (PCR) method was evaluated for detection of Legionella pneumophila in water. Two sets of primers homologous to the coding region of the L. pneumophila macrophage infectivity potentiator (mip) gene were used. Even when starting from minute amounts of L. pneumophila DNA, the double PCR products were readily detected by direct visualization in ethidium-bromide-stained agarose gels. The method was tested on 34 potable water samples from a hospital… CONTINUE READING