Nested polymerase chain reaction for detection of Ehrlichia risticii genomic DNA in infected horses.

Abstract

A nested polymerase chain reaction was developed for amplifying a 529-bp segment of the 16S ribosomal RNA gene of Ehrlichia risticii from equine buffy coat cells. Confirmation of identity of the amplified bands was accomplished by Southern hybridization and DNA sequencing. The study indicated a detection limit of > 10 copies of the target gene, and… (More)

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Cite this paper

@article{Barlough1997NestedPC, title={Nested polymerase chain reaction for detection of Ehrlichia risticii genomic DNA in infected horses.}, author={Jeffery E. Barlough and Yuta Rikihisa and J. E. Madigan}, journal={Veterinary parasitology}, year={1997}, volume={68 4}, pages={367-73} }