Nested cytochrome b polymerase chain reaction diagnostics underestimate mixed infections of avian blood haemosporidian parasites: microscopy is still essential.

@article{Valkinas2006NestedCB,
  title={Nested cytochrome b polymerase chain reaction diagnostics underestimate mixed infections of avian blood haemosporidian parasites: microscopy is still essential.},
  author={Gediminas Valkiūnas and Staffan Bensch and Tatjana A. Iezhova and Asta Kri{\vz}anauskienė and Olof Hellgren and Casimir V. Bolshakov},
  journal={The Journal of parasitology},
  year={2006},
  volume={92 2},
  pages={418-22}
}
Numerous polymerase chain reaction (PCR)-based methods have been developed and used increasingly to screen vertebrate blood samples for the diagnosis of haemosporidian blood parasites (Sporozoa, Haemosporida), but a rigorous evaluation of the sensitivity of these methods for detecting mixed infections of different haemosporidian species belonging to the same and different genera and subgenera is lacking. This study links the information obtained by nested cytochrome b PCR and traditional… CONTINUE READING
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