Nested and multiplex real-time PCR using dual-labeled probes: detecting and discriminating Mycobacterium tuberculosis complex members in cultures and animal tissues.

@article{Costa2015NestedAM,
  title={Nested and multiplex real-time PCR using dual-labeled probes: detecting and discriminating Mycobacterium tuberculosis complex members in cultures and animal tissues.},
  author={Pedro Alexandre Costa and Isabel Couto and Miguel Viveiros and Jo{\~a}o In{\'a}cio},
  journal={Methods in molecular biology},
  year={2015},
  volume={1247},
  pages={133-43}
}
Members of the Mycobacterium tuberculosis complex (MTC) are causative agents of tuberculosis (TB) in both humans and animals. In the last two decades, the accumulating knowledge of the nucleotide sequences of several genes, and of the whole genomes, of MTC members has allowed the development of novel molecular assays able to detect and discriminate between… CONTINUE READING