Nested PCR for detection and genotyping of Ehrlichia ruminantium: use in genetic diversity analysis.

@article{Martinez2004NestedPF,
  title={Nested PCR for detection and genotyping of Ehrlichia ruminantium: use in genetic diversity analysis.},
  author={D. Martinez and Nathalie Vachi{\'e}ry and Fr{\'e}d{\'e}ric Stachurski and Yane Kandassamy and Modestine Raliniaina and Rosalie Aprelon and Arona Gueye},
  journal={Annals of the New York Academy of Sciences},
  year={2004},
  volume={1026},
  pages={106-13}
}
Ehrlichia ruminantium, the agent of cowdriosis transmitted by Amblyomma ticks, presents an extensive genetic and antigenic diversity of key importance for vaccine formulation. Two means of nested polymerase chain reaction (PCR) targeting were developed to conduct molecular epidemiology studies in the Caribbean and Africa. The first used a conserved DNA fragment for detection of the pathogen in animals and vectors, and the second relied on the polymorphic map1 gene for genotyping. As compared to… CONTINUE READING

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