Nested PCR assay for detection of sugarcane grassy shoot phytoplasma in the leafhopper vector Deltocephalus vulgaris: a first report

  title={Nested PCR assay for detection of sugarcane grassy shoot phytoplasma in the leafhopper vector Deltocephalus vulgaris: a first report},
  author={S. K. Srivastava and Vijai Singh and Pramod Gupta and O. K. Sinha and Arun Baitha},
  journal={Plant Pathology},
Nymphs of Deltocephalus vulgaris, the leafhopper vector of sugarcane grassy shoot (SCGS) disease, fed on SCGS-infected and healthy sugarcane leaves, and SCGS-infected and healthy plant tissue of sugarcane cv. CoLk 8102, were examined by nested PCR using phytoplasma-specific rRNA operon primers for detection of the SCGS phytoplasma. Samples of SCGS-infected plants with symptoms and SCGS-exposed D. vulgaris nymphs yielded SCGS-exclusive DNA bands when nested PCR was performed. Negative results… 
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