Nerve outgrowth, synaptogenesis and bioelectric activity in fetal rat cerebral cortex tissue cultured in serum-free, chemically defined medium.

@article{Romijn1981NerveOS,
  title={Nerve outgrowth, synaptogenesis and bioelectric activity in fetal rat cerebral cortex tissue cultured in serum-free, chemically defined medium.},
  author={H. J. Romijn and A. M. M. C. Habets and Minke T. Mud and Pieter S. Wolters},
  journal={Brain research},
  year={1981},
  volume={254 4},
  pages={583-9}
}
Dissociated and non-dissociated cerebral cortex of fetal rat was successfully cultured in a serum-free, chemically defined medium for at least 18 days without any preincubation in serum-supplemented medium. Neurite outgrowth, synapse formation and spontaneous bioelectric activity, in its qualitative and quantitative aspects, were essentially the same as was observed in medium containing 20% heat-inactivated horse serum. 

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