Nerve growth factor induces protein-tyrosine phosphorylation.

  title={Nerve growth factor induces protein-tyrosine phosphorylation.},
  author={Pamela A. Maher},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={85 18},
  • Pamela A. Maher
  • Published 1988 in
    Proceedings of the National Academy of Sciences…
When the sympathetic nerve-like cell line PC12 is exposed to nerve growth factor (NGF), there is a rapid and transient phosphorylation of tyrosine residues in cellular proteins, as demonstrated by immunoblotting of cell extracts with high-affinity polyclonal antibodies specific for phosphotyrosine residues. Epidermal growth factor (EGF), which does not cause the morphological differentiation of PC12 cells that is produced by NGF, also induces protein-tyrosine phosphorylation. The… CONTINUE READING