Neospora caninum: in vitro culture of tachyzoites in MCF-7 human breast carcinoma cells.

@article{Lv2010NeosporaCI,
  title={Neospora caninum: in vitro culture of tachyzoites in MCF-7 human breast carcinoma cells.},
  author={Qiang Lv and Jianhua Li and Pengtao Gong and Shenyang Xing and Xichen Zhang},
  journal={Experimental parasitology},
  year={2010},
  volume={126 4},
  pages={536-9}
}
The development of Neospora caninum tachyzoites, an apicomplexan protozoan parasite, was studied in vitro using the human breast carcinoma cell 7 (MCF-7) as the host cell line. The extracellular NC-1 tachyzoites in MCF-7 cells were observed and counted daily for 6 consecutive days post-infection to establish the growth curve. The intracellular parasites were observed by acridine orange staining using Laser scanning confocal microscope. The results indicated that NC-1 tachyzoites invaded MCF-7… CONTINUE READING