Neonatal mouse gonocyte proliferation assayed by an in vitro clonogenic method.

@article{Hasthorpe1999NeonatalMG,
  title={Neonatal mouse gonocyte proliferation assayed by an in vitro clonogenic method.},
  author={Suzanne Hasthorpe and Skye Barbic and Pamela J. Farmer and John M Hutson},
  journal={Journal of reproduction and fertility},
  year={1999},
  volume={116 2},
  pages={335-44}
}
Survival and proliferation of mouse gonocytes was studied using a single cell clonogenic assay in vitro. The effect of growth factors and extracellular matrix on clonogenic development was quantitated. Fundamental requirements for growth of neonatal gonocytes included addition of fetal calf serum and coating culture wells with collagen IV alone or with added fibronectin. After 4-5 days, colonies ranged in size from four to > 128 cells, and some contained very elongated cells indicating… CONTINUE READING