Neomycin inhibition of the hammerhead ribozyme involves ionic interactions.

@article{ClouetdOrval1995NeomycinIO,
  title={Neomycin inhibition of the hammerhead ribozyme involves ionic interactions.},
  author={B Clouet-d'Orval and T K Stage and O. C. Uhlenbeck},
  journal={Biochemistry},
  year={1995},
  volume={34 35},
  pages={11186-90}
}
To investigate the properties of the neomycin-hammerhead interaction, inhibition of hammerhead activity was measured as a function of magnesium concentration and pH. The data are consistent with a simple competition between magnesium and neomycin with about five magnesium ions required to displace neomycin from the hammerhead. The pH dependence of the inhibition of hammerhead cleavage by neomycin and two related aminoglycosides was also determined. The data indicate that at least three of the… CONTINUE READING