Nematode transmission of tobacco rattle virus serves as a bottleneck to clear the virus population from defective interfering RNAs.

@article{Visser1999NematodeTO,
  title={Nematode transmission of tobacco rattle virus serves as a bottleneck to clear the virus population from defective interfering RNAs.},
  author={P B Visser and Derek J. F. Brown and Frans Th. Brederode and John F. Bol},
  journal={Virology},
  year={1999},
  volume={263 1},
  pages={155-65}
}
DI7 is a defective interfering RNA derived from RNA 2 of tobacco rattle tobravirus (TRV) isolate PpK20. Tobacco was transformed with DI7 cDNA fused to the CaMV 35S promoter. Upon infection of the transgenic plants with TRV isolate PpK20 or the serologically unrelated isolate PaY4, the transgenic DI7 RNA started to accumulate at high levels and strongly interfered with accumulation of wild-type (wt) RNA 2. When DI7 transgenic plants infected with isolate PpK20 were used as source plants in… CONTINUE READING