Negatively Stained Lipoprotein Membranes

  title={Negatively Stained Lipoprotein Membranes},
  author={Robert W. Horne and A. D. Bangham and Victor P. Whittaker},
IN a recent communication Finean and Rumsby1 have cast doubt on the reliability of the electron microscopic image obtained from negatively stained preparations of lipoprotein membrane structures (myelin, mitochondria, microsomes and red cell ghosts). Although we agree with their remarks about the need for caution in interpreting such electron micrographs, it is felt that further comment is necessary concerning some of the published literature2,3 mentioned by them. 

Structural studies of HDL and applications of EM on membrane proteins

Transmission electron microscopy was used for structure determination and for the first time the clearly discoidal shape could be shown by 3-dimendional reconstructions of ApoA1 protein dimer, indicating how it folds around a lipid bilayer in a disc-shaped structure.

A new type of pH-sensitive phospholipid

Evaluation of physical integrity of lipid bilayer under oxidative stress: application of fluorescence microscopy and digital image processing.

The one-step procedure for quantification of prooxidative effects and antioxidative protection provided by drug candidates and potential food ingredients in membranes could be easily automated for direct measurement of oxidative and oxidative effects on cellular integrity.


Liposome’s, sphere-shaped vesicles consisting of on e or more phospholipids bilayers. Today, they are a very useful reproduction, reagent, and tool in various scientif ic disciplines, including



Negatively Stained Lipoprotein Membranes: the Validity of the Image

X-ray diffraction data indicate that the dehydration of such lipoprotein systems generally leads to extensive reorganization of lipid and protein components.

Mechanically excitable receptor units in the mantle of the octopus and their connexions

  • J. Gray
  • Biology
    The Journal of physiology
  • 1960
The opportunity of a short visit to Los Angeles was taken to investigate the central connexions of mechanically sensitive receptor units in the mantle of Octopus. This preparation offers an example