Negative staining with zinc-imidazole of gel electrophoresis-separated nucleic acids.

@article{Hardy1996NegativeSW,
  title={Negative staining with zinc-imidazole of gel electrophoresis-separated nucleic acids.},
  author={Eugenio Hardy and Elder Pupo and Racmar Casalvilla and Ariadne Espinosa Sosa and Luis E. Trujillo and Eulogio L{\'o}pez and Lila Castellanos-Serra},
  journal={Electrophoresis},
  year={1996},
  volume={17 10},
  pages={
          1537-41
        }
}
Zinc and imidazole salts were applied for the detection of nucleic acids on either polyacrylamide of agarose gels. After electrophoresis, polyacrylamide gels are washed in distilled water to remove most of the residual electrophoresis reagents, then incubated in 10 mM zinc sulfate for 10 min, and subsequently immersed in 0.2 M imidazole for 3 min. As a result, zinc salts precipitate on the gel surface, except in the positions occupied by nucleic acids, which appear as transparent, colorless… CONTINUE READING

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Rapid and low-level toxic PCR-based method for routine identification of Flavobacterium psychrophilum.

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