Negative regulators of the PHO system of Saccharomyces cerevisiae: characterization of PHO80 and PHO85

@article{Uesono1992NegativeRO,
  title={Negative regulators of the PHO system of Saccharomyces cerevisiae: characterization of PHO80 and PHO85},
  author={Yukifumi Uesono and Masaya Tokai and Kazuma Tanaka and Akio Toh-E},
  journal={Molecular and General Genetics MGG},
  year={1992},
  volume={231},
  pages={426-432}
}
Both PHO80 and PHO85 genes are required to establish the repressed state of the PHO system of Saccharomyces cerevisiae. S1 nuclease protection analysis of the PHO85 transcript revealed that the PHO85 gene contains an intron at the 6th codon of the gene. Each of the fusion proteins, LacZ-Pho80 and LacZ-Pho85, was produced into Escherichia coli and used as an antigen to raise antibodies in a rabbit. Using the affinity-purified antibodies in Western blotting experiments, the PHO85 protein was… CONTINUE READING
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Mutational analysis of the SNF1 protein kinase and evidence for functional interaction with the SNF4 protein in Saecharomyces cerevisiae

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PH085, a negative regulator of the PHO system, is a homolog of the protein kinase gene, CDC28, of Saccharomyees cerevisiae

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