Negative regulation of the human polyomavirus BK enhancer involves cell-specific interaction with a nuclear repressor.

@article{Grinnell1988NegativeRO,
  title={Negative regulation of the human polyomavirus BK enhancer involves cell-specific interaction with a nuclear repressor.},
  author={Brian William Grinnell and David T. Berg and J. van der Walls},
  journal={Molecular and cellular biology},
  year={1988},
  volume={8 8},
  pages={
          3448-57
        }
}
We have examined the cell type-specific regulation of the human BK virus (BKV) enhancer. This enhancer functions efficiently in cis to activate expression from the adenovirus major late promoter in the human kidney cell line, 293, and in a monkey kidney cell line, MK2, but not in the HeLa cell line. In gel retardation migration assays, specific BKV enhancer-protein complexes could be observed by using nuclear extracts prepared from each cell line. Moreover, a unique DNA-protein complex was… CONTINUE READING
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