Negative regulation of SCFSkp2 ubiquitin ligase by TGF-β signaling

  title={Negative regulation of SCFSkp2 ubiquitin ligase by TGF-β signaling},
  author={Wei Wang and Dana Ungermannova and Jianpin Jin and J Wade Harper and Xuedong Liu},
TGF-β is a multifunctional growth factor whose best-known function is to inhibit cell growth and suppress tumor formation. TGF-β causes cells to accumulate in mid-to-late G1 phase by blocking the transition from G1 to S. It has been shown that TGF-β inhibits Cdk2-cyclin E kinase activity by promoting the binding of cell cycle inhibitor p27Kip1 to the kinase complexes. Here, we show that TGF-β treatment leads to stabilization of p27Kip1 during G1 to S transition. We found that TGF-β negatively… CONTINUE READING