Negative regulation of G1 in mammalian cells: inhibition of cyclin E-dependent kinase by TGF-beta.

@article{Koff1993NegativeRO,
  title={Negative regulation of G1 in mammalian cells: inhibition of cyclin E-dependent kinase by TGF-beta.},
  author={Andrew T Koff and Makio Ohtsuki and Kornelia Polyak and James M Roberts and Joan Massagu{\'e}},
  journal={Science},
  year={1993},
  volume={260 5107},
  pages={536-9}
}
Transforming growth factor-beta (TGF-beta) is a naturally occurring growth inhibitory polypeptide that arrests the cell cycle in middle to late G1 phase. Cells treated with TGF-beta contained normal amounts of cyclin E and cyclin-dependent protein kinase 2 (Cdk2) but failed to stably assemble cyclin E-Cdk2 complexes or accumulate cyclin E-associated kinase activity. Moreover, G1 phase extracts from TGF-beta-treated cells did not support activation of endogenous cyclin-dependent protein kinases… CONTINUE READING