Negative regulation by phosphatidylinositol 3-kinase of inducible nitric oxide synthase expression in macrophages.

@article{DazGuerra1999NegativeRB,
  title={Negative regulation by phosphatidylinositol 3-kinase of inducible nitric oxide synthase expression in macrophages.},
  author={Mar{\'i}a Jos{\'e} M D{\'i}az-Guerra and Antonio Castrillo and Paloma Mart{\'i}n-Sanz and Lisardo Bosc{\'a}},
  journal={Journal of immunology},
  year={1999},
  volume={162 10},
  pages={6184-90}
}
Triggering of the macrophage cell line RAW 264.7 with LPS promotes a transient activation of phosphatidylinositol 3-kinase (PI3-kinase). Incubation of activated macrophages with wortmannin and LY294002, two inhibitors of PI3-kinase, increased the amount of inducible nitric oxide synthase (iNOS) and the synthesis of nitric oxide. Treatment with wortmannin promoted a prolonged activation of NF-kappaB in LPS-treated cells as well as an increase in the promoter activity of the iNOS gene as deduced… CONTINUE READING