Nearest neighbor influences on DNA polymerase insertion fidelity.

  title={Nearest neighbor influences on DNA polymerase insertion fidelity.},
  author={L V Mendelman and M S Boosalis and John Petruska and Myron F. Goodman},
  journal={The Journal of biological chemistry},
  volume={264 24},
The kinetics of forming all possible single base substitution errors are measured for Drosophila melanogaster DNA polymerase alpha and avian myeloblastosis virus reverse transcriptase. Seventeen sites along bacteriophage M13 DNA are investigated so that effects of nearest neighbor base stacking on misinsertion kinetics can be evaluated. Polymerase alpha appears to be more error prone than reverse transcriptase. Polymerase alpha forms transversion mispairs at rates comparable to transition… CONTINUE READING


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