Nearest neighbor analysis of heparin: identification and quantitation of the products formed by selective depolymerization procedures.

@article{Shively1976NearestNA,
  title={Nearest neighbor analysis of heparin: identification and quantitation of the products formed by selective depolymerization procedures.},
  author={John E Shively and Hans-Ulrich Conrad},
  journal={Biochemistry},
  year={1976},
  volume={15 18},
  pages={3943-50}
}
Heparin was carboxyl-reduced with sodium boro[3H]hydride and converted to a mixture of oligosaccharides by treatment with nitrous acid at pH 2. The oligosaccharide mixture was aldehyde-reduced with sodium boro[3H]hydride and the mixture of products, labeled both in the hexoses formed in the carboxyl-reduction step and in the reducing sugars formed in the nitrous acid reaction, was separated and analyzed. The major product, L-idosyl 2-sulfate leads to anhydro-D-mannitol 6-sulfate (I), contained… CONTINUE READING