Near-perfect kinetic resolution of racemic p-chlorostyrene oxide by SlEH1, a novel epoxide hydrolase from Solanum lycopersicum with extremely high enantioselectivity.

@article{Hu2019NearperfectKR,
  title={Near-perfect kinetic resolution of racemic p-chlorostyrene oxide by SlEH1, a novel epoxide hydrolase from Solanum lycopersicum with extremely high enantioselectivity.},
  author={Bo-Chun Hu and Die Hu and Chuang Li and X. Xu and Zheng Wen and M. Wu},
  journal={International journal of biological macromolecules},
  year={2019}
}
  • Bo-Chun Hu, Die Hu, +3 authors M. Wu
  • Published 2019
  • Chemistry, Medicine
  • International journal of biological macromolecules
  • An open reading frame of sleh1, a gene encoding for a novel epoxide hydrolase from Solanum lycopersicum (SlEH1), was amplified by RT-PCR and expressed in E. coli BL21(DE3). The substrate spectrum assay showed that E. coli/sleh1 had EH activities towards all tested substrates except for racemic (rac-) 5a, and the highest enantiomeric ratio (E > 200) towards rac-2a, retaining (R)-2a with 99.1% ees and 49.2% yields and affording (R)-2b with 89.8% eep and 46.7% yieldp. Besides, E. coli/sleh1 also… CONTINUE READING
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