Near-atomic cryo-EM imaging of a small protein displayed on a designed scaffolding system.

Abstract

Current single-particle cryo-electron microscopy (cryo-EM) techniques can produce images of large protein assemblies and macromolecular complexes at atomic level detail without the need for crystal growth. However, proteins of smaller size, typical of those found throughout the cell, are not presently amenable to detailed structural elucidation by cryo-EM… (More)
DOI: 10.1073/pnas.1718825115

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