Near-IR resonance Raman spectroscopy of archaerhodopsin 3: effects of transmembrane potential.

@article{Clair2012NearIRRR,
  title={Near-IR resonance Raman spectroscopy of archaerhodopsin 3: effects of transmembrane potential.},
  author={Erica C Saint Clair and John I. Ogren and Sergey Mamaev and Daniel Russano and Joel M Kralj and Kenneth J Rothschild},
  journal={The journal of physical chemistry. B},
  year={2012},
  volume={116 50},
  pages={14592-601}
}
Archaerhodopsin 3 (AR3) is a light driven proton pump from Halorubrum sodomense that has been used as a genetically targetable neuronal silencer and an effective fluorescent sensor of transmembrane potential. Unlike the more extensively studied bacteriorhodopsin (BR) from Halobacterium salinarum, AR3 readily incorporates into the plasma membrane of both E. coli and mammalian cells. Here, we used near-IR resonance Raman confocal microscopy to study the effects of pH and membrane potential on the… CONTINUE READING