Nature of the displaceable heme-axial residue in the EcDos protein, a heme-based sensor from Escherichia coli.

@article{Gonzlez2002NatureOT,
  title={Nature of the displaceable heme-axial residue in the EcDos protein, a heme-based sensor from Escherichia coli.},
  author={Gonzalo Gonz{\'a}lez and Elhadji M. Dioum and Craig M Bertolucci and Takeshi Tomita and Masao Ikeda-Saito and Myles R. Cheesman and Nicholas J. Watmough and Marie-Alda Gilles-Gonzalez},
  journal={Biochemistry},
  year={2002},
  volume={41 26},
  pages={8414-21}
}
The EcDos protein belongs to a group of heme-based sensors that detect their ligands with a heme-binding PAS domain. Among these various heme-PAS proteins, EcDos is unique in having its heme iron coordinated at both axial positions to residues of the protein. To achieve its high affinities for ligands, one of the axial heme-iron residues in EcDos must be readily displaceable. Here we present evidence from mutagenesis, ligand-binding measurements, and magnetic circular dichroism, resonance Raman… CONTINUE READING