Naturally occurring masked antibodies in murine sera recognize a component of the mitotic spindle apparatus.

@article{Munns1986NaturallyOM,
  title={Naturally occurring masked antibodies in murine sera recognize a component of the mitotic spindle apparatus.},
  author={Tom Munns and M Kathryn Liszewski and Sandra K. Freeman},
  journal={Biochemical and biophysical research communications},
  year={1986},
  volume={136 1},
  pages={
          145-50
        }
}
Antibody activities previously masked in autoimmune MRL and normal Balb/c mice were expressed by briefly subjecting their sera to acidic (pH 2.0) or alkaline (pH 12.0) environments. An enzyme-linked immunosorbent assay (ELISA) revealed that these pH-expressable immunoglobulins reacted with specific nucleotide-BSA antigens (primarily 5'-AMP, -GMP, -TMP) but not with single (ss)- or double (ds)-stranded DNAs or with unconjugated BSA. ELISA analysis of pH-expressed antibodies purified via GMP-BSA… CONTINUE READING