Naturally occurring active N-domain of human angiotensin I-converting enzyme.

  title={Naturally occurring active N-domain of human angiotensin I-converting enzyme.},
  author={Peter A. Deddish and Jean Wang and B Michel and Patrick W. Morris and Nicholas O. Davidson and Randal A. Skidgel and Ervin G. Erd{\"o}s},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={91 16},
Angiotensin I-converting enzyme (ACE, kininase II) is a single-chain protein containing two active site domains (named N- and C-domains according to position in the chain). ACE is bound to plasma membranes by its C-terminal hydrophobic transmembrane anchor. Ileal fluid, rich in ACE activity, obtained from patients after surgical colectomy was used as the source. Column chromatography, including modified affinity chromatography on lisinopril-Sepharose, yielded homogeneous ACE after only a 45… CONTINUE READING