Naturally-arising amino acid polymorphisms of HIV-1 Nef that differentially modulate downregulation of HLA-A and HLA-B molecules

Materials and methods Plasma HIV RNA-derived Nef clones (one per patient) were isolated from 45 chronically-infected subjects and inserted into the pNL43 proviral vector. Recombinant viruses were prepared and used to infect the HLA-ldeficient cell line 721.221 ectopically expressing either HLA-A*24 or HLA-B*35. Following infection, cell-surface HLA-I… CONTINUE READING