Natural and Genetically Engineered Proteins for Tissue Engineering.

@article{Gomes2012NaturalAG,
  title={Natural and Genetically Engineered Proteins for Tissue Engineering.},
  author={S{\'i}lvia Gomes and Isabel B. Leonor and Jo{\~a}o F. Mano and Rui Lu{\'i}s Reis and David Lee Kaplan},
  journal={Progress in polymer science},
  year={2012},
  volume={37 1},
  pages={
          1-17
        }
}
To overcome the limitations of traditionally used autografts, allografts and, to a lesser extent, synthetic materials, there is the need to develop a new generation of scaffolds with adequate mechanical and structural support, control of cell attachment, migration, proliferation and differentiation and with bio-resorbable features. This suite of properties would allow the body to heal itself at the same rate as implant degradation. Genetic engineering offers a route to this level of control of… 
Recombinant silk like fusion proteins as next generation matrices for tissue engineering
There is a growing need for tissue transplantations that cannot be met with current biomedical methods. Tissue engineering (TE) is a new interdisciplinary field that aims to construct viable tissues
Advances in Protein-Based Materials: From Origin to Novel Biomaterials.
TLDR
This review addresses an up-to date review on the novel, protein-based biomaterials used for biomedical field including tissue engineering, medical science, regenerative medicine as well as drug delivery.
Naturally and synthetic smart composite biomaterials for tissue regeneration.
TLDR
In-depth, recent developments concerning smart composite biomaterials available for delivery systems of biofactors and cells and scaffolding matrices in tissue engineering are reviewed.
Biological materials and molecular biomimetics - filling up the empty soft materials space for tissue engineering applications.
TLDR
This work highlights the recent application of Next Gen sequencing technologies for the characterization of several protein-based natural biopolymers, a technique which circumvents this research bottleneck in molecular biomimicry of these model protein systems.
Interactions of cells with silk surfaces
Polymers are often employed in tissue engineering to replace damaged extracellular matrix (ECM). During the last few decades silk proteins have been extensively investigated concerning their use as
Osteoinductive recombinant silk fusion proteins for bone regeneration.
Protein polymers provide a unique opportunity for tunable designs of material systems due to the genetic basis of sequence control. To address the challenge of biomineralization interfaces with
Soft tissue engineering and microbial infections
TLDR
Tissue engineering applications could benefit from the design of multifunctional biomaterials, assuring simultaneously the scaffold function, as well as the release of antibiotics, growth factors, and other bioactive molecules, in order to prevent infections and therefore to accelerate optimal tissue regeneration.
Recombinant protein blends: silk beyond natural design.
TLDR
Advances in recombinant DNA-mediated protein production and functionalization approaches are reviewed, with a focus on hybrids or combinations of proteins; recombinant protein blends or 'recombinamers'; and the potential biomedical applications of fibrous protein recombinamers are highlighted.
Designing ECM-mimetic materials using protein engineering.
TLDR
This brief review demonstrates how fundamental knowledge gained from structure-function studies of native proteins can be exploited in the design of novel protein-engineered biomaterials.
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 299 REFERENCES
PROTEIN TEMPLATES IN HARD TISSUE ENGINEERING.
TLDR
The importance of proteins as templates for regeneration and repair of hard tissues as well as the potential of peptide based nanomaterials for regenerative therapies are highlighted.
Natural origin biodegradable systems in tissue engineering and regenerative medicine: present status and some moving trends
TLDR
In this review, the most studied and promising and recently proposed naturally derived polymers that have been suggested for tissue engineering applications are described and their blends with synthetic polymers are analysed, with special focus on polysaccharides and proteins.
Protein-engineered biomaterials: nanoscale mimics of the extracellular matrix.
TLDR
A modular design strategy to synthesize protein-engineered biomaterials that fuse together multiple repeats of nanoscale peptide design motifs into full-length engineered ECM mimics that are ideal substrates for reductionist biological studies of cell-matrix interactions, for in vitro models of physiological processes, and for bio-instructive scaffolds in regenerative medicine therapies.
Testing the utility of rationally engineered recombinant collagen-like proteins for applications in tissue engineering.
TLDR
This work genetically engineer a collagen-like protein consisting of tandem repeats of the D4 domain (mD4 collagen) to provide a basis for rational engineering of similar proteins for a variety of biomedical applications.
Nanoscale engineering of biomimetic surfaces: cues from the extracellular matrix
TLDR
The development of new nanotechnologies such as photo- or electron-beam nanolithography, polymer demixing, nano-imprinting, compression molding, or the generation of TiO2 nanotubes of defined diameters (15–200 nm), has opened up the possibility of constructing biomimetic surfaces with a defined nanopattern, eliciting tissue-specific cellular responses by stimulating integrin clustering.
Heparinized hydroxyapatite/collagen three-dimensional scaffolds for tissue engineering
TLDR
Hydxyapatite three dimensional porous scaffolds were produced and heparin was incorporated in order to accomplish sustained delivery of a growth factor of interest namely, bone morphogenetic proteins (BMP-2).
Cell adhesion on artificial materials for tissue engineering.
TLDR
Tissue engineering has been developed to meet increasing demand for safe, functional and easy available substitutes of irreversibly damaged tissues and organs, characterized by so-called three dimensional porous or scaffold-like architecture promoting attachment, growth and differentiation of cells inside the material.
Biomimetic materials in tissue engineering
Biomaterial matrices are being developed that mimic the key characteristics of the extracellular matrix, including presenting adhesion sites and displaying growth factors in the context of a
Potential applications of natural origin polymer-based systems in soft tissue regeneration
TLDR
The characteristics, properties, and compatibility of the resulting materials investigated in the last 10 years, as well as commercially available matrices or those currently under investigation are the subject matter of this review.
Controlled drug delivery in tissue engineering.
TLDR
Although these platforms represent a first attempt to mimic the complex temporal and spatial microenvironment presented in vivo, an increased symbiosis of material engineering, drug delivery technology and cell and molecular biology may ultimately lead to biomaterials that encode the necessary signals to guide and control developmental process in tissue- and organ-specific differentiation and morphogenesis.
...
1
2
3
4
5
...