Natural Endophytic Occurrence of Acetobacter diazotrophicus in Pineapple Plants

@article{TapiaHernndez2000NaturalEO,
  title={Natural Endophytic Occurrence of Acetobacter diazotrophicus in Pineapple Plants},
  author={A. Tapia-Hern{\'a}ndez and M. R. Bustillos-Cristales and T. Jim{\'e}nez-Salgado and J{\'e}sus Caballero-Mellado and Luis Ernesto Fuentes-Ram{\'i}rez},
  journal={Microbial Ecology},
  year={2000},
  volume={39},
  pages={49-55}
}
The presence of endophytic Acetobacter diazotrophicus was tested for pineapple plants (Ananas comosus [L.] Merr.) grown in the field. Diazotrophic bacteria were isolated from the inner tissues of surface sterilized roots, stems, and leaves of pineapple plants. Phenotypic tests permitted the selection of presumptive nitrogen-fixing A. diazotrophicus isolates. Restriction fragment length polymorphisms (RFLPs) of small subunit (SSU) rDNA using total DNA digested with endonuclease SphI and with… CONTINUE READING