Natural Endophytic Occurrence of Acetobacter diazotrophicus in Pineapple Plants

  title={Natural Endophytic Occurrence of Acetobacter diazotrophicus in Pineapple Plants},
  author={Armando Tapia-Hern{\'a}ndez and Mar{\'i}a Del Roc{\'i}o Bustillos-Cristales and T. Jim{\'e}nez-Salgado and Jesús Caballero-Mellado and Luis Ernesto Fuentes-Ram{\'i}rez},
  journal={Microbial Ecology},
A bstractThe presence of endophytic Acetobacter diazotrophicus was tested for pineapple plants (Ananas comosus [L.] Merr.) grown in the field. Diazotrophic bacteria were isolated from the inner tissues of surface sterilized roots, stems, and leaves of pineapple plants. Phenotypic tests permitted the selection of presumptive nitrogen-fixing A. diazotrophicus isolates. Restriction fragment length polymorphisms (RFLPs) of small subunit (SSU) rDNA using total DNA digested with endonuclease SphI and… 
Identification of culturable endophytes in 'Champaka' pineapple grown in an organic system
‘Champaka’ pineapple plants at fruiting stage associate with endophytes related to α, β and γ Proteobacteria, after plantlets inoculation or not with the diazotrophic bacterium Burkholderia silvatlantica (AB 219a), a characteristic for nitrogen-fixing bacteria.
A N2-fixing endophytic Burkholderia sp. associated with maize plants cultivated in Mexico.
In the frame of a survey of potentially endophytic N2-fixing Burkholderia associated with maize in Mexico, its country of origin, the soil of an indigenous maize field near Oaxaca was studied and it was hypothesized that strains of this species had developed a sort of primitive symbiosis with one of their host plants, teosinte, which persisted during the domestication of teosintse into maize.
Can a N2-fixing Gluconacetobacter diazotrophicus association with sugarcane be achieved?
It is concluded that a N-2-fixing sugarcane - G. diazotrophicus association is not easily achievable, being primarily limited by a lack of infection.
Burkholderia, a Genus Rich in Plant-Associated Nitrogen Fixers with Wide Environmental and Geographic Distribution
The results obtained show that many diazotrophic Burkholderia isolates analyzed showed phenotypic and genotypic features different from those of the known N2-fixing species B. vietnamiensis as well as from B. kururiensis, a bacterium identified in the present study as a diazOTrophic species.
Detection of nifH Sequences in Sugarcane (Saccharum officinarum L.) and Pineapple (Ananas comosus [L.] Merr.)
Bacteria with nifH sequences homologous to those of bacteria in the genera Bradyrhizobium, Serratia, and Klebsiella may be promising candidates for predominant endophytic diazotrophs in sugarcane and pineapple.
Isolation and Characterization of Acetobacter, Herbaspirillum and other Endophytes from Sugarcane and other Plants
  • 2016
This investigation was carried out to isolate Acetobacter, Herbaspirillum and other plant growth promoting endophytes from different plants. These plants included sugarcane, mango, banana, pineapple,
Isolation and screening of nitrogen fixing endophytic bacterium Gluconacetobacter diazotrophicus GdS25
Gluconacetobacter diazotrophicus is an endophytic bacterium first isolated from the sugarcane growing regions of Brazil (Cavalcante and Dobereiner, 1988). It was widely studied and used as a model
Population Dynamics of Gluconacetobacter diazotrophicus in Sugarcane Cultivars and Its Effect on Plant Growth
The results show the importance of the sugarcane variety for the persistence of the plant–bacteria interaction, and it could explain the different rates of BNF estimated among Sugarcane cultivars.


Coffea arabica L., a new host plant for Acetobacter diazotrophicus, and isolation of other nitrogen-fixing acetobacteria
Endophytic diazotrophic bacteria may be more prevalent than previously thought, and perhaps there are many more potentially beneficial N2-fixing bacteria which can be isolated from other agronomically important crops.
Contributions of the Bacterial Endophyte Acetobacter diazotrophicus to Sugarcane Nutrition: A Preliminary Study
Interestingly, when N is not limiting, both wild typeand mutant-inoculated plants showed increased growth compared to uninoculated control plants suggesting other possible beneficial effects of A. diazotrophicus to sugarcane in addition to nitrogen fixation.
Root colonization and systemic spreading of Azoarcus sp. strain BH72 in grasses
PCR and Western immunoblotting, using primers specific for eubacteria and antibodies recognizing type-specific antigens, indicated that strain BH72 could colonize rice plants systemically, probably mediated by longitudinal spreading through vessels.
Population dynamics of endophytic bacteria in field-grown sweet corn and cotton
Results indicate that endophytic bacteria are natural inhabitants of internal regions of roots and stems and that the endophytes may arise from both seeds and soils.
Limited Genetic Diversity in the Endophytic Sugarcane Bacterium Acetobacter diazotrophicus
The results of the genetic analysis indicate that the genetic structure of A. diazotrophicus is clonal, with one largely predominant clone.
A Nitrogen-Fixing Endophyte of Sugarcane Stems (A New Role for the Apoplast)
This demonstrates the presence of an N2-fixing endophyte living in apoplastic fluid of plant tissue and also that the fluid approximates the composition of the endophytes's optimal culture medium.
Genetic Structure of Acetobacter diazotrophicus Populations and Identification of a New Genetically Distant Group
A total of 55 isolates of Acetobacter diazotrophicus recovered from diverse sucrose-rich host plants and from mealybugs associated with sugarcane plants were characterized by the electrophoretic mobilities of 12 metabolic enzymes, finding more genetic diversity in isolates from Brazil than in those from Mexico.